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`Notice
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`complete, reliable or fit for specific purposes. Critical decisions, such as commercially relevant or
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`DESCRIPTION JP2007078676
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`[0001]
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`The present invention relates to analytical apparatus using the analysis device for optically
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`analyzing a biological fluid, and the analysis device, and more particularly, the component
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`measurement of the biological fluid with an optical analyzer in the analysis device to be used,
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`relates to how to collect the solution components or solid components, in particular to a method
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`for collecting a plasma component or a blood cell component in the blood.
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`[0002]
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`Conventionally, as a method for optically analyzing a biological fluid, a method of analysis using
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`a microdevice is known that the formation of the liquid flow path.
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`Microdevice, using the rotary apparatus is capable of controlling the fluid by utilizing the
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`centrifugal force of the sample solution metering, the separation of the solid components, since it
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`is possible to carry out transfer distribution of separated fluid, etc. , it is possible to perform
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`various biochemical analyzes.
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`[0003]
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`By utilizing the centrifugal force as a method of transferring the sample solution, as shown in
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`Figure 23, a large fluid chamber 8 1, along with being connected to the large fluid chamber 8 1,
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`radially outward relative to the large fluid chamber 8 1 a measuring chamber 82 which is
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`arranged, with the overflow chamber 83 connected to the measuring chamber 82, a receiving
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`chamber 84 disposed radially outward relative to the measuring chamber 82, the liquid in the
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`receiving chamber 84 from the metering chamber 82 and capillary connecting means 85 for
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`feeding, there is a rotation analysis device with.
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`[0004]
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`Capillary connecting means 85 includes a siphon 86 which has a capillary structure, elbow—like
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`bent portion of the siphon 86, the center of rotation analysis device, so as to be radially
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`innermost point substantially the same distance in the measuring chamber 82 and are
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`positioned, for the capillary force during rotation of the rotary analysis device is smaller than the
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`centrifugal force, the interface liquid / air has the same axis as the axis of rotation analysis device
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`and the measuring from the center of rotation analysis device the measuring chamber 82 in
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`agreement with the rotating cylindrical body shape having a radius of length equal to the
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`distance to the radially innermost point of the chamber 82 is filled with the sample solution, the
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`excess sample solution overflow chamber 83 It flows into.
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`[0005]
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`When stopping the rotation analysis device, the sample solution filled in the metering chamber
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`82 flows into the capillary tube connecting means 85 in the capillary force, by rotating again,
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`liquid siphon is started, present in the metering chamber 82 is discharged to the receiving
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`chamber 84 (Patent Document 1).
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`[0006]
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`In this case, if the sample solution has a solid component, and subjected to centrifugal separation
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`in the measuring chamber 82 or receiving chamber 84, after precipitating the solid component is
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`allowed to connect the capillary with a siphon structure in a portion located radially inwardly
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`and if, the only solution components in the sample solution, it is possible to transfer to the next
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`process.
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`Hei 5—508709 JP
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`[0007]
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`However, in the conventional configuration, after centrifugation, by simply adjusting the position
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`for connecting the capillary tube, only the solution components in the sample solution can be
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`transferred, but since the solid component is precipitated in the outer circumferential direction In
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`the transfer by siphoning, and had a problem that it is impossible to transport the solid
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`component or a high concentration of the solid component solution.
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`[0008]
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`Also, in the case of transferring only the solution components in a capillary having a siphon
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`structure, along with remaining solution stopped rotation, flows back into the capillary tube,
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`since the solution in the capillary is transported again by the next rotation, and the liquid The
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`amount of variation and, also it had a problem that affects the accuracy of measurement, such as
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`by the influx of solid component into the capillary.
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`[0009]
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`The present invention is intended to solve the above conventional problems, the solid
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`component, or a high concentration can perform the transfer of the solid component solution,
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`and, upon transferring the portion of the sample solution, the remaining solution is chase rear in
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`and an object analysis device can be prevented to come to flow, and to provide an analytical
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`device using the same.
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`[0010]
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`In order to solve the above conventional problems, the analysis device according to a first aspect
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`of the present invention, a sample solution to be analyzed is housed in the sample solution
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`analyzing device which can transport the internally, the sample solution the solution component,
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`into a solid component, the separation chamber for separated using a centrifugal force generated
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`by rotation of the analysis device, a part of the solid component separated in said separation
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`chamber is transferred, a holding channel for holding this, provided between the separation
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`chamber and the holding channel, the overflow channel which is connected by a connection
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`passage for transferring the sample solution of said separation chamber, are provided, wherein
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`the separated in the separation chamber is present in the connection path solution components,
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`after preferentially flows into the overflow channel, the solid component separated in the
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`separation chamber, through said connecting passage The filled in the overflow channel,
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`subsequently, the portion of the solid component which has flown into the overflow channel is
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`held is introduced into the holding channel preferentially, it is those wherein the Te .
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`[OOIH
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`Analysis device according to claim 2 of the present invention, the analysis device according to
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`claim 1, the overflow chamber in which the sample solution filled in the overflow channel in is
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`discharged, further comprising the overflowing fluidic chamber is connected via a coupling part
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`is connected to the overflow channel, transferred from the connection path of the sample
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`solution into the overflow channel is intended to be done by a capillary force, the overflow
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`between the channel and the overflow chamber, the opening area of
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`the overflow channel in the
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`coupling portion is larger than the opening area of
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`the holding channel, it is characterized in.
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`[001%
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`Analysis device according to claim 3 of the present invention, the analysis device according to
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`claim 1, the overflow chamber in which the sample solution filled in the overflow channel in is
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`discharged, further comprising the overflowing fluidic chamber is connected via a coupling part
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`is connected to the overflow channel, transferred from the connecting channel of the sample
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`solution into the overflow channel is intended to be made by feeding the overflow from the air
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`hole provided in the chamber, the suction pump, the overflow channel and the air in the
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`connecting passage is sucked, by a pressure difference that can be by the suction, the solution
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`components present in the connection path, preferentially The can is transferred to the coupling
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`portion between the overflow chamber of the overflow channel, it is characterized in.
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`[0013
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`Analysis device according to claim 4 of the present invention, the analysis device according to
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`claim 1, wherein the retaining channel and communicating, with the solid component, a mixing
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`chamber for mixing the dilute solution and the reagent solution When the mixing chamber and in
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`communication, and a diluent storage chamber for accommodating the diluting solution or the
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`reagent solution, further comprising, that it is characterized.
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`[0014]
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`Analysis device according to Claim 5 of the present invention, the analysis device according to
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`claim 1, wherein the retaining channel is to weigh the solid component by the volume of the flow
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`path, are those wherein the .
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`[0015
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`Analysis device according to Claim 6 of the present invention, a sample solution to be analyzed is
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`housed in the sample solution analyzing device which can transport the inside, a liquid chamber
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`for containing the sample solution, the Liquid storage chamber and communicating, by the
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`capillary force is transferred to the sample solution from the liquid storage chamber, and the
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`retention channel for retaining a portion of the sample solution, a sample of the holding flow
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`path that is transported by an external force a liquid holding chamber for holding the solution,
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`when rotating the device for the analysis axis direction, is located outside the said axis with
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`respect to the liquid chamber, through a capillary channel having a siphon structure a sample
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`overflow chamber which is connected to the liquid chamber, and with it it is obtained by the
`features.
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`[001m
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`Analysis device according to Claim 7 of the present invention, the external force is a centrifugal
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`force generated by rotation of the analysis device, it is obtained by the features.
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`[OOlfl
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`Analysis device according to Claim 8 of the present invention, in the analysis device of claim 6,
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`wherein the external force is generated by the introduction of gas from the air hole provided in
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`the liquid separation position of the holding channel is a pressure, that it is obtained by the
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`features.
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`[0018]
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`Analysis device according to Claim 9 of the present invention, the analysis device according to
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`claim 6, wherein the liquid containing chamber, the sample solution, a solution component and a
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`solid component, the rotation of the analysis device It is one having a separation chamber for
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`separating using a centrifugal force generated, the holding channel is to transfer only the
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`separated solution component of the sample solution from the separation chamber via the
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`connecting passage , it is one that has been characterized by.
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`[0019]
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`Analysis device according to Claim 10 of the present invention, in the analysis device of claim 6,
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`wherein the retaining passage, which its metering the solution to be retained by the volume of
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`the flow path, it was characterized by It is.
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`[0020]
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`Analysis device according to Claim 1 l of the present invention, in the analysis device of claim 6,
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`wherein the liquid containing chamber, the sample solution, a solution component and a solid
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`component, the rotation of the analysis device are those having a separation chamber for
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`separating by using the centrifugal force generated, the holding channel for retaining a portion
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`of the separated solid component in the separation chamber, the separation between the
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`retaining channel provided between the chambers, said the separation chamber and the overflow
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`channel which is connected by a connecting passage, and an overflow chamber which is
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`connected to the overflow channel, further comprising, that is one which has been characterized
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`by is there.
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`[002M
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`Analysis device according to Claim 1 2 of the present invention, in the analyzing device of claim
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`1 1, wherein the retaining channel and the overflow channel is, have a capillary size that generate
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`capillary flow of the liquid, the the opening area of
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`the overflow channel in the connecting
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`portion between the overflow chamber of the overflow channel is, the larger than the opening
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`area of
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`the holding channel, that it is obtained by the features.
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`[002%
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`Analysis device according to Claim 13 of the present invention is retained in the analysis device
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`according to claim 1 1, is connected to the separation chamber, the sample solution of the
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`separation chamber, a portion of the separated solution component a solution component
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`holding channel for the filling solution component holding channel in the solution components is
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`maintained, the solution components and the reagents to be mixed / reacted, and measuring the
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`absorbance or turbidity of the mixed solution a measuring cell for, further comprising, that it is
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`obtained by the features.
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`[002%
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`Such analyzer to Claim 14 of the present invention, there is provided an analytical apparatus for
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`analyzing device described is mounted in claim 2, the analyzing device comprises a rotation
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`driving means for rotating about its axis, The separation chamber the sample solution is to
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`separate the sample solution by rotating the analysis device in a state of being housed in a
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`solution component and a solid component, and by stopping the subsequent rotation of the
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`analysis device, the separation chamber The inflow solution components to the connecting
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`passage, the capillary force is transferred in preference to the coupling portion between the
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`overflow chamber of the overflow channel, after which the solid component of the separation
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`chamber from the above by a capillary force was transferred into the holding channel, and that it
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`is re-rotating the analysis device, the overflow channel and filled solution component to the
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`coupling portion and the solid component, is discharged to the overflow chamber, it is
`characterized in It is.
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`[0024]
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`Analysis device according to Claim 1 5 of the present invention, there is provided an analytical
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`apparatus for analyzing device described is mounted in claim 3, a rotation driving means for
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`rotating the analysis device around the axis, for the analysis a pump for sucking the liquid in the
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`device and provided with a coupling mechanism for connecting the air hole of the analysis device
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`and the pump, with the sample solution accommodated in the separation chamber, said analysis
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`By rotating iodide devices, the sample solution was separated into a solution component and a
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`solid component, followed, after stopping the rotation of the analysis device, by the coupling
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`mechanism is connected to the air hole of the overflow chamber , by sucking air in the pump, it is
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`transported in favor of a solution component, which has flowed into the connecting passage to
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`the bonding portion between the overflow chamber of the overflow channel, further, the coupling
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`part is After being filled, the and by connecting the connecting mechanism to the air hole of the
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`connecting retention chamber for retaining passage, and by sucking air in the pump, the solid
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`component of the separation chamber into the holding channel is transferred, after the holding
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`channel is filled with a solid component, and is disengaged with the coupling mechanism, it is
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`possible to make re—rotating the analysis device, the overflow channel and that is filled at the
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`junction solution component and a solid The components, said discharging the overflow
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`chamber, it is characterized in.
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`[0025
`
`Analysis device according to Claim 16 of the present invention, there is provided an analytical
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`apparatus for analyzing device described is mounted in claim 7, further comprising a rotation
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`drive means for rotating the analysis device around its axis, the By rotating the analysis device in
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`which the sample solution is contained in a solution storage chamber, thereby transferring the
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`sample solution to the outer periphery of the liquid storage chamber, after which, by stopping
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`the rotation of the analysis device, the by capillary force from the liquid storage chamber, held by
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`sucking the sample solution into the holding channel, further, the capillary channel having a
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`siphon structure that is connected to the sample overflow chamber by the capillary force from
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`the liquid containing chamber the sample solution is held by sucking the said By rotating the
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`analysis device, the sample solution filled in the holding flow path is transferred to the liquid
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`holding chamber, the liquid chamber of the sample solution, the capillary the siphon structure of
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`the road, the sample overflow chamber is discharged into, it is characterized in.
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`[002m
`
`Such analyzer to Claim 1 7 of the present invention, there is provided an analytical apparatus for
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`analyzing device described is mounted in claim 8, a rotation driving means for rotating the
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`analysis device around the axis, for the analysis and a gas introduction mechanism for
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`transferring the liquid in the device, by rotating the analysis device in which the sample solution
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`is contained in a solution storage chamber, and then transferring the sample solution to the outer
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`periphery of the liquid storage chamber, Then, the analysis by stopping the rotation of the
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`device, held by sucking the sample solution into the holding channel by capillary force from the
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`liquid chamber, and further said sample overflow by capillary forces from the liquid containing
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`chamber chamber and is held by sucking the sample solution into the capillary flow path having
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`a siphon structure that is connected, the gas introducing mechanism, and an air hole provided in
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`the liquid separation position of the holding channel, the liquid containing chamber inner is
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`connected to the air hole is provided in a circumferential portion, wherein the gas introduction
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`mechanism via the air hole and by introducing a gas, a sample solution filled in the holding flow
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`path, and the liquid separation position of In in isolation was transferred to the liquid holding
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`chamber, the liquid chamber of the sample solution by a siphon structure of the capillary channel
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`and is discharged into the sample overflow chamber, it is characterized in.
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`[OOZfl
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`Analysis device according to the present invention, and according to the analysis device, the solid
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`component after centrifugation, or a high concentration of solid component solution, it is
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`possible to transfer only the required amount.
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`Furthermore, upon transferring the portion of the sample solution, the remaining solution can be
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`prevented that the incoming flows at chase rear, it is possible to improve the measurement
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`accuracy of the analysis device.
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`[0028]
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`Below, we will explain the embodiment of the analysis device of the present invention in detail
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`with reference to the accompanying drawings.
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`(Embodiment 1) Hereinafter, a first aspect of the present invention, the second aspect, and billing
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`analysis device 101 according to the first embodiment corresponding to the section 5 and the
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`measuring apparatus 1000 using the same, FIGS. 5 is used to explain.
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`[0029]
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`Figure 1 is a schematic view showing a configuration of the analysis device 101 according to the
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`first embodiment of the present invention.
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`In Figure 1, the analysis device 101 according to the first embodiment, in a substrate 1 having a
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`micro channel 4a, the 4b, a flat substrate 2, an the both substrates 1 and 2, the adhesive layer 3
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`to be bonded to each other It is configured.
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`In addition, 5 is the reaction solution filled in the microchannel 4a of the micro-channel formed
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`by laminating the two substrates 1 and 2.
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`[0030]
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`Microchannels 4a, 4b formed on the substrate 1, the micro—channel pattern of irregularities have
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`been produced by injection molding, the sample solution to be analyzed, is injected into the
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`analysis device 101, the micro—channel 4a , and it becomes possible to transfer the sample
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`solution within the through 4b.
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`[003M
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`In the first embodiment, by irradiating the transmitted light 6a in the microchannel 4a, optically
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`analyzing the reaction state of the sample solution and a reagent to be tested.
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`During the measurement, the reaction solution 5 prepared by reacting the sample solution and
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`the reagent in the microchannel 4a is filled, the reaction solution 5, since the absorbance at a
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`rate of reaction of the sample solution and the reagent is changed, the micro light source 6 is
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`irradiated with light transmitted through the channels 4a, and by measuring the light quantity of
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`the transmitted light by the light receiving section 7, it is possible to measure the change in the
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`amount of light transmitted through the reaction solution 5, thereby analyzing the reaction
`conditions to be I can.
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`[003%
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`In the first embodiment, the thickness of the substrate 1 and the substrate 2, which are formed
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`by 1mm~5mm, this is not particularly limited, the microchannel 4a, if 4b are capable of forming
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`thick good.
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`In addition, the shape of the substrate 1 and the substrate 2 is also not particularly limited, the
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`shape in accordance with the intended use, for example, disk-shaped, fan shape, sheet shape,
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`plate shape, rod shape, and other molded products of complex shape It is possible to take the
`form of such.
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`[003%
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`In the first embodiment, as the material of the substrate 1 and the substrate 2, moldability, high
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`productivity, the use of plastics in terms of low cost, which is glass, silicon wafers, metals,
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`ceramics, etc., If it joined possible materials and not particularly limited.
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`[0034]
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`In the first embodiment, the substrate 1 and the substrate 2 having micro-channels 4a, the 4b,
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`when transferring the solution by utilizing the capillary phenomenon, the micro—channels 4a, in
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`4b hydrophilic treatment is performed as required and, if Hodokose this, it is possible to facilitate
`fluid transfer reduces the viscous resistance of the microchannel.
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`[0035
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`In the first embodiment, due to the use of plastic materials, although performing a hydrophilic
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`treatment to the surface, or with a hydrophilic material such as glass, a surfactant at the time of
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`molding, a hydrophilic polymer, such as a parent of silica gel the hydrophilizing agent is then
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`added the material surface, such as a powder but may also be or is rendered hydrophilic.
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`As a method for hydrophilic treatment, plasma, corona, ozone, surface treatment method and
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`using the active gas such as fluorine, and a surface treatment with a surface active agent.
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`Here, the contact angle with water and the hydrophilicity refers to less than 90 degrees, more
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`preferably less than the contact angle of 40 degrees.
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`[003@
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`In the first embodiment, so it is by using an adhesive is bonded to the substrate 1 and the
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`substrate 2, the substrates 1 and 2, which are joined in a joining method such as fusion bonding
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`or anodic bonding depending on the materials used It may be in.
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`[003W
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`Figure 2 is a schematic diagram showing a configuration of the analyzer 1000 to the analysis
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`device 101 according to the first embodiment is mounted.
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`In Figure 2, the analysis device 101 according to the first embodiment, is mounted on the motor
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`102 is a rotary drive means of the analyzer 1000 of Embodiment 1, by driving the motor 102,
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`the analysis iodide device 101 can be rotated around the axis.
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`The analysis device 101 can then transfer the liquid in the device using a centrifugal force
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`generated by rotation can be or is centrifuged.
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`In the first embodiment, although wearing the analyzing device of the disc—shaped in the
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`analyzer, a fan shape, or those of other shapes, it is also possible to mount a plurality of
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`simultaneously.
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`[0038]
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`In addition, the present analysis apparatus 1000, while the analysis device 101 is rotated in C
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`direction by the motor 102, is irradiated toward the analysis device 101 with laser light from the
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`laser light source 103.
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`A laser light source 103, is screwed to the feed screw 105 driven by a traverse motor 104, a
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`servo control circuit 106, so that the laser light source 103 can be located at any measuring
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`point, and drives the traverse motor 104, You can move the laser light source 103 in the radial
`direction.
`
`[0039]
`
`The upper part of the analysis device 101, of the laser beam irradiated from the laser light
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`source 103, a photodetector 107 that detects the amount of light transmitted through the
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`analysis device 101, the adjustment circuit 108 that adjusts the gain of the output of the
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`photodetector 107 , you want to save the amount of transmitted light signal processing circuit
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`1 10 for processing the outputs the A / D converter 109, A / D conversion for A / D conversion
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`data of the adjustment circuit 108, obtained by the amount of transmitted light signal processing
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`circuit 1 10 data memory 1 13, it has a display unit 1 1 2 to display the results CPU1 1 1, and
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`analyzed to control these.
`
`[0040]
`
`Then, micro—channel configuration of the analysis device 101 of the first embodiment, and the
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`sample solution transfer process in detail explained.
`
`Figure 3 is a plan view illustrating a microchannel structure of the analysis device 101 according
`to the first embodiment.
`
`In addition, Fig. 4 (a), (b) a diagram for explaining the injection / separation process of the
`
`analysis device 101 according to the first embodiment, FIGS. 5 (a), (b), (c) is present weighing of
`
`the analysis device 101 of Embodiment 1, and is a diagram for explaining the filling process of
`the measurement cell 28.
`
`[0041]
`
`3, 4, as shown in FIG. 5, the microchannel configuration of the analysis device 101 according to
`
`the first embodiment, the liquid storage chamber for injecting / accommodate the volume of the
`
`sample solution required for analyzing 9 When the sample solution, into a solution component
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`and a solid component, the separation chamber 10 for the separated using a centrifugal force
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`generated by rotation of the analysis device, a part of the solid component separated by the
`
`separation chamber 10 is transferred, a holding channel 13 for holding this, between the holding
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`channel 13 and the separation chamber 10, overflow channel, which is connected by a
`
`connecting passage 1 1 for transferring the sample solution in the separation chamber 10 1 2,
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`and the overflow chamber 15 to fill sample solution overflow channel 12 is discharged to retain
`
`the solid component filled in the holding channel 13 and is mixed / reacted with the solid
`
`component and the reagent Te, the absorbance of the mixed liquid, and a turbidity or a
`
`measuring cell 28 for measuring the number of cells in.
`
`[0042]
`
`Here, in the first embodiment, in the measurement cell 28, a reagent for reacting with the solid
`
`component is supported.
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`Furthermore, although omitted in the first embodiment, between the holding channel 13 and
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`measurement cell 28, and a reagent reaction chamber for reacting the sample solution with the
`
`reagent, the stirring chamber or the like for carrying out the agitation is provided that may be.
`
`[0043]
`
`In the first embodiment, the liquid storage chamber 9, the separation chamber 10, overflow
`
`chamber 1 5, although the depth of the measurement cell 28 is formed by 0.3mm~2mm, which, or
`
`the amount of the sample solution and the absorbance Conditions for measuring is adjustable
`
`(optical path length, measured wavelength, reactive concentration of the sample solution, the
`
`type of reagent and the like) by.
`
`[0044]
`
`Liquid storage chamber 9 is connected with the separation chamber 10, a pre—metered amount of
`
`the sample solution was injected / housing from the inlet 8 as shown in FIG. 4 (a), by rotating the
`
`present analysis device 10 1 By generating the centrifugal force, as shown in FIG. 4 (b), it is
`
`possible to transfer the sample solution into the separation chamber 10.
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`[0045]
`
`Furthermore, in the first embodiment, although not provided a metering function for causing the
`
`predetermined amount holding the sample solution to a separation chamber 10, in order to
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`reduce the sample solution before injection step, metering the sample solution to a separation
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`chamber 10 Weighing function, for example, when the analyzing device is rotated, configured
`
`such as to drain the excess fluid to the overflow chamber via an overflow channel from the liquid
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`surface position to hold the required amount of the separation chamber, or separation of The
`
`capillary channel formed by communicating from the chamber to the outside of the analysis
`
`device is provided, the sample solution is sucked by the capillary force of the capillary channel,
`
`and the sample solution was metered by volume of the capillary channel by centrifugal force The
`
`sample solution in the capillary flow passage may be provided a configuration as to transfer to
`
`the separation chamber.
`
`[0046]
`
`In the first embodiment, although connected with the liquid chamber 9 by the separation
`
`chamber 10 to the same depth, due to the sample solution at the time of injection is prevented
`
`from flowing into the separation chamber 10, the air holes in the separation chamber 10 It is
`
`provided, and may be connected by capillary channel depth 50 ,u m~200 ,u m.
`
`[0047]
`
`Separation chamber 10, from the radially outermost position of the separation chamber 10, and
`
`is connected with the overflow channel 12 through the connecting passage 1 1.
`
`Connecting passage 1 1, 0.5mm~2mm the passage width, but forms a depth 50 [,L m~200 [,L m,
`
`which is a sample solution by the capillary force generated during the rotation stop of the
`
`analysis device, in connection passage 1 1 as long as it can be filled, and is not intended to be
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`particularly limited thereto.
`
`[0048]
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`23-10-2015
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`15
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`

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`In the configuration of the first embodiment, by rotating the analysis device 101, the sample
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`solution from the liquid storage chamber 9 when they were transported to the separation
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`chamber 10, the connecting position of the connecting passage 1 1 and the overflow channel 1 2
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`Beyond, as the sample solution does not flow out from the separation chamber 10, from the
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`amount of the sample solution to be pre-weighed, the size of the separation chamber 10, the
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`connecting positions of the connecting passage 1 1 and the overflow channel 12 is optimized Te,
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`it is necessary to prevent the outflow of the sample solution.
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`In the first embodiment, in this regard, the connecting passage 1 1, since the separation chamber
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`10 are formed to the inner peripheral position than the liquid surface when holding a quantity of
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`the sample solution, in Figure 4 (b) As shown, the sample solution is transferred from the liquid
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`storage chamber 9 by the centrifugal force is held in the separation chamber 10 and the
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`connecting passage 1 1.
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`[0049]
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`The sample solution held in the separation chamber 10, and by rotating a few minutes at high
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`speed, as shown in FIG. 4 (b), a solution component 3 1, it is possible to separate the solid
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`component 32.
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`For example, in the case of blood, set the rotational speed to 4000rpm~6000rpm, and by rotating
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`one minute to 5 minutes, and plasma, in the blood or high hematocrit blood (the ratio is high
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`blood blood cells), be separated it can.
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`[0050]
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`Separation chamber 10 is connected to the holding channel 13 through the connecting passage
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`1 1 and the overflow channel 12, the overflow channel 1 2 and the overflow chamber 15 located
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`in the outer peripheral direction from 322% flow channel 12 The ligated retention channel 1 3 is
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`coupled with the measurement cell 28 in the outer circumferential direction than the holding
`channel 1 3.
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`[005M
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`23-10-2015
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`16
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`

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`The depth of the holding channel 13 and the overflow channel 1 2 is formed in 50 [.L m~200 [.L m,
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`when stopping the rotation of the analysis device, by capillary forces, the separated solid
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`component or a high concentration in the separation chamber 10 to fill in the solid component
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`solution holding channel 13 and the overflow channel 1 2.
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`[005%
`
`At this time, first, all of the solution component 3 1 which is separated in the connecting passage
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`1 1 is transferred to the overflow channel 1 2 by capillary but force, since the depth of the
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`overflow chamber 1 5 is formed deeply , solution component 31 does not transfer to the overflow
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`chamber 1 5, is held at the junction 30 between the overflow channel 1 2 and the overflow
`chamber 1 5.
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`Then, solid component 32 are separated by the connecting passage 1 1 and the separation
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`chamber 10 is transferred to the holding channel 13 via the overflow channel 1 2.
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`[005%
`
`In the configuration of the first embodiment, as shown in FIG. 4 (b), when centrifuged in the
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`separation chamber 10 to the sample solution, the inner peripheral portion of the connecting
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`passage 1 1 which connects the separation chamber 10 and the overflow channel 1 2 To the
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`solution component 31 is present, the solution component 3 1 flows into the retaining channel 13
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`as it is, the concentration of solid components decreases, it becomes a factor cau