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`U1
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`10
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`CROSS~REFERENCE T0 RELATED APPLICATIONS
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`This ie5acontinuatiom.application.of International Patent
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`ApplieationNo.PCT/JP2014/O75598filedSeptemberzs,2014,which
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`Claims the benefit of priority’to Japanese Patent.App1icatiot1Nou
`
`2013«203338 filed September 30, 2013. The full contente of the
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`International Patent Application are incorporated herein by
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`reference.
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`Technical Field
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`BACKGROUND
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`The present disclosure relates to a working chamber, such
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`asanieolator,acfleenbench,oreioebinet,usedforearegenerative
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`medical experiment enviionmentel device and a pharmaceutical
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`experiment environmental device,iflwhichziworkieuuiie inserted
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`into a box Shaped work space to conduct work.
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`Description of the Related Art
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`In Japanese Patent Application Laidwopen Publication No.
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`20
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`2011—177091, an isolator is disclosed that can.prevent dust from
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`being mixed into cells and the like and can prevent dust
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`from
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`effecting<miltuzezworke eucilae c ll.manipuletion. This isolator
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`supplies gas downward from above a working chamber by an air
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`current control neane, and discharges the gas in the working
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`Chamber from discharge hole portions provided on‘a work plate.
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`The present disclosure is to provide aiworking‘chamber that
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`improves workability.
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`3O
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`A working chamber according to the present disclosure
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`SUMMARY
`
`
`
`IX.)
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`includes: a box»sheped body case including a work space in an
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`interior thereof and an insertion portion,
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`in a front eurfsc.
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`thereof,
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`to which a worker’s arm is inserted; a supoly unit
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`configured to eupp1}r,
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`into the work epeoe, gas in an exterior of
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`the body case through.e supply filter provided at the upp-or front
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`oftheworkepeceinthebodyoaee;alouverthetguideegeesugplied
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`into the work Space by the supply unit to a back surface side of
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`3..)
`the worksspace after the gas gasses through the supply fi ter;
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`and an illleinating means pO'itioned lower than the louver and
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`10
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`in front ofthe louver in the work soaoe,
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`in an upper part of the
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`work spa.e in the body'oaee, at least one surface of the louver,
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`which fiaoes the illuminating means, being a reflection surface,
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`cherfeature‘oftqtprosentln.thtlonmillh
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`appare
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`front dee oriptione of
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`the present specification aod, of
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`the
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`15
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`aooom anyi ng dxrawinge.
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`BRIEF DESCRlPTION OF THE DRAWINGS
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`Eor more thorough unders tan.ding of the presen.t invention
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`20
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`and.e&vantagee thereof, the following deeo rlpti-om.ehould.be read
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`in conjunction with the accompanying drawings,
`
`in which:
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`Fig.l.isEiperepeotivexmKWJillustratingéulis oletor‘eystem
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`acoordioy to an embodiment 1;
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`Fig.
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`2
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`is a perspective View fill 11streting the isolator
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`system when an incubator is mounted thereto according to the
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`embodiment 1;
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`Fig,
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`3 is a front View of the isolator system according to
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`the embodiment 1;
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`Fig. 4 is a 4-4 croeewseotionel View oi a glove box in Fig.
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`bi
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`ua
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`
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`to
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`Fig.
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`5
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`is an explanatory View showing a course of an
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`i1].umineting light from an illuminating unit according to the
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`embodinweht 1;
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`a Q Q)SQ m S(1) R (D U QQ;Q} iment; and
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`cross~seotions1 vieurof the glove hoxzeocording
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`Fig.
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`7
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`is an exp1' anetory View sh.owing a course of an
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`gm:
`11 umineting'light fireflies.iiiumineting‘unit aocordinthJanother
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`embodiment.
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`10
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`DETAEQED DESSERT PTIQN
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`At lee‘t the following detaiis will become apparent from
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`descriptions of the present specificatiion.andtfifthe accompanying
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`drawings”
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`Hexeinefteer, embodiments m711 he described in detail with
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`reference to drawings as necessary. However, a description in
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`m‘re detailed than necessary may be omitted For exampie,
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`detailed descripti one of we11 known matters and the repeated
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`descriptions of We stant ia11y t11e same configurations may be
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`omitted. This istxsprevent the tol1owing’des'ription fronabeing
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`unnecessarily redundant
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`than necessary, and to facilitate the
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`understanding of a person skiiied in the art,
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`Note that the inverters_provid.e the accompanying drawings
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`and the following descriptions to help a person skilled in the
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`art fully“undetstandifleapresent disclosure, andauxanot intended
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`to limit the sub.ect matters desori.bed in the claims thereby.
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`20
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`25
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`Embodiment 1)
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`An.isolstorssystem 3.0G mlili‘he described hereinafter as an
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`example of a working chamber in an embodiment 1 with reference
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`to Figs.
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`1 to 5.
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`The isolator system 100 in the embodiment 1 is a device
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`
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`m
`eon ignred to perform, for example, work for cell culture,
`.fi.I
`.L
`in a steri 12zed environment.
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`manipulation, observation, etc.,
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`2-0 m
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`
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`(I) P4C .
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`e that sterilization means an act of killing microorganisms,
`1
`‘11.«L
`and toe like,
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`to bring a state closer to a sterile
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`83
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`U}
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`envirtsnnent.
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`Note that,
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`in the present embodiment, it is assumed that
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`the Z axis is an axis along a vertical direction in which the
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`isolator system 100 is pr vided is a standing manner, and a
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`direction toward the upper side is +Z direction and a direction
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`10
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`toward the lower side (downward) is *2 diiec
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`L).
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`on”
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`It is assumed
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`that the Y axis is an axis talong a direction.§wpendieular°to the
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`front surface and the back surfiaoe of the isolator system 100,
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`and a direction from the front surface, where omen ings for
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`conducting work in the interrioor of a work space are provided,
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`15
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`toward the back surface opposite to the front surface is ~Y
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`directios., and5;dirtttias.£iomtimibaok,surfm e towwar
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`the front
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`surface is +Y’direotion”
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`It is assumed that the X.axis is an axis
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`alongeadireotionperpendieulartxntheleftandrightsidesurfaoes
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`wheniseeanronithe front, andaidireoti nEron:the.leftsideém1rfaoe
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`20
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`toward the right side srrfaoe when seen from the front is +X
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`direction, and,a«direotioo.frow1the right side surface toward.the
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`1eft side surface is ”X direction.
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`[1. Isolator System]
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`As entire configuration of the isolator system 100
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`25 will be described with reference to Figs” 1 to 4. Fig.
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`1 is a
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`perspective View illustrating an isolator system 100 accordilig
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`to an embodiment 1. Fig. 2 is a perspective View illustrating
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`the isolator system 160 when an incubator 200 is mounted thereto
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`according to the embodiment 1. Fig.
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`3 is a front View of the
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`Lu (I)
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`isolator system 100 according to the embodimeot 1. Fig. 4 is a
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`
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`U}
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`414 cross—sectional View of a glove box 110 in Fig. 3,
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`As illust ratedrin.Fig‘ l, the isolator syStefillOGtaccording
`.J\
`.a
`to the embodiment l includes: a clove box 110; a centrifuge unit
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`120; an observation unit 130; a sterilization unit 140; an air
`.
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`conditioning uz1i t 153; a control unit 160 (see Fig 1}; a pass
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`box 170; and an air conditioning unit 180.
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`lustrated in Fig, 2,
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`in the isolator system 100,
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`the
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`incubator 200 is mounted.to the side surface opposite to the side
`r
`1
`ctrrace to which the pass box 170 is provided in the glove box
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`.
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`10
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`1...)
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`10. Tlie incubator 200 includes a storage chamber anct shown
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`\l
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`in the interior thereof. The storage chamber is a chamber for
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`storing a culture, and is partitioned as a space to restrain
`1
`.
`uA.
`invas1on from the exterior for example, by
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`rec angular‘parallelepipedwshapedlxncbody; The storage chamber
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`is partitioned with, for e amcl e, stainless steel plates, The
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`incubator 200 is demountably configured with respect to the
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`isolatcr system 100, Thus, culture can be coitrolled in each
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`incubator‘zOO. For‘example,aidediceted.incubator‘ZOQ canlxaused
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`for each donor,
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`thereby restraining occurrence of failures such
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`[U CD
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`as mixwup of culture.
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`As illustrated in Fig.
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`l,
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`the glove box 110 includes a
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`substantially beX sh.aped work space A,
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`formed in tile inte ric
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`therecE, that is isolated.Erom the surroundings to cond.uctwrk
`fl
`.
`1
`in.the sterinized environment. A.detailed.ccnfiguraticn.w1ll b
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`(D
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`n1
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`described later, The centrifuge unit 120 is provided below the
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`glove hex 110, and can be connected from the work space A.
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`’Fhe
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`centrifuge unit
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`120
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`includes,
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`in the interior
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`thereof,
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`a
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`centrifuge configured to centrifuge a sample to work with.in the
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`workspaceit Theobservatiorunit130‘mssrovicmibel1 theglove
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`30
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`box 110,
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`and can be connected from t.e work space A.
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`The
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`
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`1
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`observation unit 130 includes,
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`in the interior thereof,
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`an
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`observation device configured to obsierve a ssmp1 e to work with
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`in.the work.spaoe.A. Further, the observation.unit 130 includes:
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`a
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`lifting mechanism. capable of
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`lifting and lowering the
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`ohservationwievioegmrwidediritheinteriorthereofi;andxahandle,
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`provided in the exterior, with which the lifting mechanism is
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`operatet.
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`A worker operates the 11 ft11ng mechanism using the
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`handle, so that the observation device can he moved up into the
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`work.
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`space A) when;
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`the observation. device is used, and the
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`1C1
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`servetion.device cam be hous ed.intthe observation.unit 130 when
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`the observation unit 130 is not‘usedo The sterilize tionfiunit 140
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`.J.
`is provided below the glove box 11G,
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`to supply a steirilizing
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`subs tanoe eioh as hyd;ogen peroxi.de into the glove box 110 The
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`sterilisation unit 146 in the present embodiment is oonfigin ed
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`to spray sterilizing mist, obtained by converting sterilizing
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`liouid into mis through.a nozzle p1ovided.in the glove boxl110,
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`to steriiize the interior thereof.
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`The air conditioning unit
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`50 is provided.above the glove
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`box 110, and is configured to control the air conditioning in the
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`interiorthereof. Thebes Cundlt-Ofl ngtrn1;].501nclude sasupply
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`uni“ 150a and a discharge unit lEOb. The air oondi tioning unit
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`1:0 is configured to supply gas into the work space A using the
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`supply’uni 5150s, and d1 soharge the gas in.the werRLspaceA using
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`the discharge unit 150 b.
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`As illustrated.in Fig 3,
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`the control uniit 160 issprovided
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`above the glove box 110 and the pass box 170, and is configured
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`tcacontrol the<operations of theaieviees suciiaas the sterilisation
`‘
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`unit 140 and the air sondition1m1g unit 159.
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`The pass box 170 is provided to the side surface of the glove
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`30
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`box 110 to allow the worker to bring a work obj eat from the exterior
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`
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`into the interior of the work space A In the interior of the
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`pass box 170 , a conveying space .3 is formed in which the work ohj ect
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`is temporarily stored.
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`the conveying space E has airtightness
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`with respect to surrounding environment. Before the work object
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`is inserted into the work space A from the exterior, the work object
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`is sterilised in the cvonveying space E An opening for moving
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`the work. object is provided to the side surface of the pass box
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`170 and faces an opening provided to the side surface of the glove
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`foo}: 110, so that the glove box 110 and the pass box 170 are fixed.
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`10
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`In this way, the work spaceA and the conveying.»space E commmicate
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`with each other while maintaining airtightness. The opening of
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`the pass box 170 has an operiahle/closeble door installed thereto.
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`The door can eparate the conveying space E3 from the work space
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`A while maintaining airtightness . The air conditioning unit 180
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`is provided at tne upp-or portion of the pass box 170, and is
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`configured to control the air conditioning in the conveying space
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`B .111 the pass bex 1'70.
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`[2 Home Box
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`5...:
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`The configuration of the glove box 110 will he described
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`N 0
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`with reference to Fig. 4. Fig. 4 is a 4M1: cross-sectional View
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`of a glove box in Fig. 3. As ill1strat ed in Fig. 4, in the glove
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`box 110,
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`the hox—shaped body case is configured with a front
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`surr ace pl ate 111 having a plurality of front surface openings
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`:4
`H2 which are insert ion portions for worker’s hands, a hack
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`nrface pltae .1113, a top surface plate 21.14;, a bottom surfacesplate
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`115, and left and right side surface pl at s. The glove box 110
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`includes,
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`in the interior thereof: a work plate 3.16 on which work
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`is conducted; and a partition1ng plate 137 provided to the 1hack.
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`surface side of the glove box. The hos: shaped work space A that
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`is a space for conducting work is formed with the front surface
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`
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`plate 111,
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`the top sur ace plate 114,
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`....A
`the work pl
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`.‘
`ate 115,
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`the
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`partitioningplatell7,andtheleftelerightsidesurfaceplates.
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`fiflnglovekxntllfiisaicompartmehtthat3A3formedvflxfilairtightnese
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`so as to restrain.baeterial invasion from the exterior.
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`In the
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`glove box 110 of the present emboeiment,
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`the work plate 116,
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`the
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`par‘it ioni1mg plate 117,
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`the bottom sorta oe plate 115,
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`the b'ok
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`
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`{F2 h: H111 a
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`oe plate 113,
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`the top surface plate 114, and the left and
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`H l". LC}.
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`a. y
`t side surface plates are configured with stainless steel
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`(E)
`g...)p at s, which is easily cleaned and sterilized.
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`'16;
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`10
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`Gloves(notehown)aterespectivelynmuntedtotheplutallty
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`of front surface openings 11.2. The front surface plate 111 is
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`openable/clo sable about a hinge provideé an EApper end thereof
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`serving as an axis. Thus,
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`the ront surt’ ace of the glove box 13.0
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`can.be opened/closed” Openings foz‘mountifig the pass box 170 and
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`theinoubatorZOOateformmjonleftandrightsidesurfaceplates.
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`The pass box 170 is mounted. to the opening on the right side surface
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`plate of tile glove box 13.0, and the inouba toI 200 is mounted to
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`the operii;
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`on the left side surface plate thereof. A worker
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`conducts work in the interior of the work.spaee A througb,gloves
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`20
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`at the time of working.
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`A bottom surface duet D1 is formed.hetween the work plate
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`116 and the bottom surface plate 115 as a discharge path thr ugh
`
`which.the gea in the work.spacefa is discharged. Further, a back
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`surface duit D2 is formed.be een.the partitionlrg slate 117 anfi
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`N U?
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`the back surface plate 113 as a discharge path through which the
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`gas ih the work space A is discharged” The bottom surface duet
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`D1 and the back surface duet D2 communicate with each other. The
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`gas supplied,fromLhe inlet 151 flows substatxt lly1ln.aejlrettiou
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`Indlta'td.oy a:rows in the figure, and.passes through the bottom
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`surface duct D1 and.the back surface duet D2 to be diseharged.from
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`
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`the outlet 156.
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`In this way,
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`the gas flow can he stabilized in
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`the work space A.ahd contamination can be suppressed.
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`The top surface plate 114 in the gloW box 110 is provided
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`with an inlet 151 and an outlet 156. The inlet 151 is dis*osao
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`at the upper front of the work space A, and the outlet 156 is
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`disposed at the upper rear thereof. The gas is supplied into the
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`work space A from the inlet 151, ahd the gas in the work space
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`A is discharged from the outlet 156.
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`In the glove box 113, a
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`particulate trap filter 152 such as an HEDA filter is mounted to
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`10
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`the inlet 151 to secure the stterile environment in L,he interior,
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`and the gas is supplied through the particulate trap fiilter 152
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`U":
`into the 'love box 110” Further, aparticul ate t1ap tilt r1 7
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`such as the HE?A filter is menot
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`”L
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`the ozltlet 15 6, and.the gas
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`in the glove box 110 is discharged.from the interior'of the glove
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`box 110 through the particulat
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`trap filter 15”].
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`C311 the work space
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`A.side of the top surface plate 114 in the glove box 110, a louver
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`153 is provided that guides the gas supplied into the work space
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`A.hy the supply unit 150a to the back surface side of the work
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`space A,
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`that is,
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`tc the partitioning plate 117 side, after the
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`gas passes tl1rW=gh the particulW e trap filter 152, The upper
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`portion of the louver 153 is fixed to the top surface plate 114
`..I
`1
`so as to allow the lower portion of the louver 153 to be closer
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`to theahaok:surfaoe side-than.the upper‘portiontthereof.
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`In.other
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`words,
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`the louver 13d 1s inclined to the back surface side from
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`the upper portion toward the lower portion“ Further,
`1
`surface of the lou.ver 15 3 is mirror- 'ihiehed to he a reflection
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`the front
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`surface.
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`An illuminati ng‘unit 118 is provided at the upper front of
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`the work.spaoe.A in the glove box 110 for easily conducting works
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`by brightly illuminating the interior of
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`the work space A,
`
`25
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`30
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`
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`1Q
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`particularly,
`
`the work plate 116, The illuminating unit 118 is
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`positioned lower than the louver 353ah6.1h.fr0ht of the louver
`a
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`153 in the work space A.
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`in the isolator system lOG,
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`the
`
`in:
`illuminating unit 1 fl is provided to the upper front side of the
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`wo..k space A in a horizontal direction.
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`{3. Effects, etc.
`
`:....r
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`Fig.
`
`5
`
`is to: explanatory*'view =3 owing ea course Cd? an
`
`illuminatihg light from :15illuminating unit 118 according to
`
`the embodiment 1.
`
`As stated shove,
`
`in the present embodiment,
`
`the isolator
`
`system 100 (vorking chamber)
`
`includes: the box shaped glove box
`
`110 (body case) having the work space A in the interior thereof
`
`é.)r
`and the front surface openings 112 ( neertlon portions) on the
`
`frontsurfacethereof,txawhichaiwother’sarmsere t;o beihserted;
`
`the supply unit 150a (supply part) configured0 supply the gas
`
`outsie the glove box llD into the work space A through the
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`particulate trap filter 152 (supply filter} fot‘air'tutplyy which
`
`is provioed at the upper front of the work space A in the glove
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`hox.llo;-the louV'er‘lSBt at guides the gas supplied.into the work
`
`1 s
`
`pace A by the supply unit 150a to the back surface side of the
`
`work space A after the gass passes through the particulate trap
`
`filter 152; and the illuminating unit 118 (illuminating means)
`
`positioned lower than the louver 153 eiid in front of the louver
`
`153 in the work space A in the upper poxrti Ch of the work space
`
`A in the glove box 110, and at least one surface of the louver
`
`153, which faces the illuminating unit 118,
`
`is a reflection
`
`Thus, not only the gas flow in the work space A is stabilized
`
`contaminationieasuppres edlniallowingtjmzlouver‘153tx3guide
`
`the gas supplied from the upper front of the work space Ato the
`
`10
`
`in: U}
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`20
`
`N '1)“:
`
`30
`
`
`
`ll
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`back surface side,as illustra:. ed11 Sig. 51, but also workability
`
`or" workers can be improved by reflecting the light at
`
`the
`
`illuminating unit 118 by the louver 153 to illuminate the work
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`plate 116, as illustrated in Fig. 5.
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`On the other hand,
`
`if. a member for collecting the light of
`
`the illuminating unit 118 to the work plate 116 is provided
`
`separately, the cost is inLcreased due to the i norease of the number
`
`ot parts, andalso workability is deteriorated since the werk space
`
`A becomes narrower.
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`However, the isolator system 100 111 the present embodiment
`
`is provided with the louver 153,
`
`and thus both suppressing
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`contamination and illuminating the work plate 116
`
`can be
`
`colfigured by one member.
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`Further, the iselator sysstem 100 includes : the particulate
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`trap filter 157 (discharge fiilter) for discharging gas, which is
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`provVided at the upper rear of the glove box 110;
`
`the discharge
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`unit 1501::- (discharge part) that. discharges air
`
`3.1.
`
`:1 the work space
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`*0 the outside through the particulate "rap f lter 157; and the
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`U1
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`(3
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`1": (f1
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`partitioning plate 3.1717hieh is provided on the b surface side
`
`20
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`of the glove box 1.
`
`1..:
`
`.-’
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`to form a ventilation passage between the
`
`back surface plate 113 of glove box 110 and the partitioninc pl“ te
`
`117 and partition air currents into supply air and discharge air.
`
`Consequently, the isolator system 100 can separate the air
`
`current supplied into the work space from the air current to be
`
`25
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`discharged from the work. space A This can further suppress
`
`centamihation.
`
`(Other Embediment)
`
`As described above, the embodiment: has been descrihed as
`
`an exampleatten thesislique disclosed inhe present appliea tion.
`
`LO C)
`
`dowever, the present
`
`isolosed technique is not limited thereto,
`
`
`
`but
`
`ie applicable to an embodiment
`
`in which modifications,
`
`replacements, additions, omission; and the like are made as
`
`appropriate. For,her,
`
`the components described in the above
`
`embodiment 1 can be combined to configure a new embodiment”
`
`Then, other embodiments will hereinafter be exemplified.
`
`Note that,
`
`the same oomponente as those in the embodiment 1 will
`
`be designated by the same reference numerals as those in the
`
`embodim_mnt 1, for convenience’ sake,
`
`In the embodiment 1,
`
`the glove box 110 has been described
`
`10
`
`15
`
`as en.exemple of the body oeee. Tie glove box 110 is not limited
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`to a body case for en isolator in which work is conducted in the
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`mrk. space A through gloves, The present dieoloeed. technique is
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`useful.aleo foraioleenix,1iatrEioabinet,hevingth2front surface
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`openings 112 to which no glove is mounted”
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`In short,
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`the body
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`case may be the one in vm] .h.ges is supplied into the wori space
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`A.to adjuet an environment of the work epaoe.A,
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`in an experimental
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`environmept dewice nwhi oh a worker ineerte hie/her hand into
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`the work epeoe A to conduct work in the interior thereofo
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`Further,atopboardfeoingtheworkplatellCmavbeprov1d
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`at thhe upper part of the work space A that is formed.in the glow
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`box 110 in the embodiment 1.
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`In this case, it is necessary for
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`the top board.to be provided.with.hole portione through.w11oh.the
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`supplied gas pee see. Fig. 6 is e erosewseotiozlal View of a gloJe
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`box 190 according to another embodiment. The glove box 190 shown
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`in Fig 6 includes a top board 119 having a plurality of hole
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`portions. Fig. 7 is an explanatory View showing a course of en
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`lluminating light from the illuminatino unit 118 ac cording to
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`another embodiment“
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`lustrated.h1Fig.ch thelouverlSB ie d.epoee
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`,etween
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`thetoyboerdllQendthepartioulatetimpfilteileaforwlreupply
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`
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`13
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`U}
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`143
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`20
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`The illuminating unit 118 is dispesed below the tap beard 119,
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`and the light at
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`the illuminating unit 118 passes through a
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`plurality of hole perti OflS te lu‘-t a refl ectien eu1wf ee of the
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`leuverlSB.
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`'heh, therefleetedlightreflectejhythexef1eetien.
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`surface passes
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`through,
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`t e p.31ura1.ity‘ ef hQ1e pertione and
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`illuminates the werk plate 116 of the werk space A.
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`Censequently, as illustrated in Fig. 6,
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`the gas supplied
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`from the upper fr 0th 015' the werk space A by the 1011ve.153 is guided
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`to the back surface side, and alse the gas passes threugh the
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`plurality of hole pertiens ef the tap board 119. Ceneequehtly,
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`the gas ie uniformly supplied into the work space A. Thee,
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`the
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`air current in the werk space A is stabilized.and contaminatien
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`.4
`is suppressed. Eurther, as iluetrated in F'e. 7, altheugh the
`.,
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`intensity 017 light ef the illuminating unit 118 is reduced because
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`the light passes threueh the plurality 0f hole pertiohe of the
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`tap board 119, it is possible that the illmminating light that
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`has 1aassed through the top boardl 19 is reflectefi by the louver
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`153toilluminatethewerkplatellé. Thiscanimprevewerkahility
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`of workers.
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`Nete that,
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`the shape of the leave: 153 is not limited to
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`a plat plate shape, but may'he a shape in whith a sectionai Shape
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`on Y Z axes plane is a pretr11ded shape te the werk space side,
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`that is, the center part hetween.the upper part and.the lower part
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`at the louver 153 may have a shape swelling'to the i11umineting
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`unit 118 side, Thus, it hecemes pessihle to allow the reflected
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`1 e
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`light t0 he reflected over a wider range, so t
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`at a wider range
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`cm1thewerkplate116canbeilluminated. Thiscanfurtherimprove
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`workahi lity at werkerso
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`