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`/J,H,/’
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`US. Patent Application Serial No.: 12/530,555
`Response filed June 25, 2015
`Reply to 0A dated March 25, 2015
`
`AMENDMENTS TO THE CLAIMS:
`
`This listing of claims will replace all prior versions, and listings, of claims in the
`
`application:
`
`Listing of Claims:
`
`Claim 1 (Currently amended): An incubation apparatus to incubate culture targets such as
`
`cells, embryos or microorganisms in an incubation chamber, the incubation apparatus including:
`
`a gas concentration sensor which detects a first gas concentration in the incubation chamber;
`
`a controller which controls the supply of a first gas into the incubation chamber on the basis
`
`of an output of the gas concentration sensor;
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`a standard gas supply device which can supply the gas concentration sensor with a standard
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`gas which has been previously adjusted to an actually used gas concentration or a gas concentration
`
`close thereto, wherein the standard gas and the actually used gas include the first gas and a second
`
`gas; and
`
`a passage control device which can connect the incubation chamber or the standard gas
`
`supply device to the gas concentration sensor selectively,
`
`wherein the controller is programmed to maintain the concentration of the standard gas,
`
`supply the standard gas to the gas concentration sensor by the standard gas supply device, and on the
`
`basis of an output of the gas concentration sensor and the concentration of the standard gas at the
`
`moment, calibrate the actually used gas concentration of the gas concentration sensor by controlling
`
`the passage control device,
`
`

`

`US. Patent Application Serial No.: 12/530,555
`Response filed June 25, 2015
`Reply to 0A dated March 25, 2015
`
`wherein the controller is connected to at least a first valve which is connected to a first pipe
`
`extending out of the incubation chamber, connected to a second valve which is connected to a second
`
`pipe connecting the standard gas suppiy device to the second valve, and connected to the gas
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`concentration sensor,
`
`wherein the standard gas includes at least carbon dioxide gas, oxygen gas and nitrogen gas.
`
`Claim 2 (Previously presented): The incubation apparatus according to claim 1, further
`
`including
`
`an outside air supply device which supplies outside air to the gas concentration sensor,
`
`wherein the outside air supply device is composed of a third valve and a third pipe
`
`connecting air to the third valve, and the controller is connected to the third valve and configured to
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`control supply of the outside air to the gas concentration sensor by the third valve of the outside air
`
`supply device, and on the basis of an output of the gas concentration sensor at the moment,
`
`is
`
`configured to perform zero-point calibration of the gas concentration sensor.
`
`Claim 3 (Previously presented): The incubation apparatus according to claim 1 or 2, further
`
`including a detachable cylinder in which the standard gas is hermetically contained, the standard gas
`
`being supplied from the cylinder to the gas concentration sensor by the passage control device.
`
`Claim 4 (Previously presented): The incubation apparatus according to claim 1 or 2, further
`
`including a connection opening to connect a pipe which supplies the standard gas to the gas
`
`concentration sensor.
`
`

`

`US. Patent Application Serial N0.: 12/530,555
`Response filed June 25, 2015
`.
`Reply to 0A dated March 25, 2015
`
`Claim 5 (Previously presented): The incubation apparatus according to claim 1 or 2, wherein
`
`the controller is configured to calibrate the actually used gas concentration at preset time intervals.
`
`Claim 6 (Previously presented): The incubation apparatus according to claim 1, wherein the
`
`gas concentration sensor is capable of detecting the first gas concentration and a third gas
`
`concentration in the incubation chamber, and
`
`wherein the controller which controls the supply of the first or third gas into the incubation
`
`chamber on the basis of an output of the gas concentration sensor;
`
`wherein the standard gas and the actually used gas include the first gas, the second gas and
`
`the third gas.
`
`Claim 7 (Previously presented): The incubation apparatus according to claim 6, wherein the
`
`gas concentration sensor including,
`
`a first gas concentration sensor which detects the first gas concentration in the incubation
`
`chamber, and
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`a third gas concentration sensor which detects the third gas concentration in the incubation
`
`chamber.
`
`Claim 8 (Previously presented): The incubation apparatus according to claim 7, wherein
`
`the first gas concentration sensor and the third gas concentration sensor are connected in series.
`
`

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