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`US. Patent Application Serial No.: 12/530,555
`Response filed June 25, 2015
`Reply to 0A dated March 25, 2015
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`AMENDMENTS TO THE CLAIMS:
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`This listing of claims will replace all prior versions, and listings, of claims in the
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`application:
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`Listing of Claims:
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`Claim 1 (Currently amended): An incubation apparatus to incubate culture targets such as
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`cells, embryos or microorganisms in an incubation chamber, the incubation apparatus including:
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`a gas concentration sensor which detects a first gas concentration in the incubation chamber;
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`a controller which controls the supply of a first gas into the incubation chamber on the basis
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`of an output of the gas concentration sensor;
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`a standard gas supply device which can supply the gas concentration sensor with a standard
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`gas which has been previously adjusted to an actually used gas concentration or a gas concentration
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`close thereto, wherein the standard gas and the actually used gas include the first gas and a second
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`gas; and
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`a passage control device which can connect the incubation chamber or the standard gas
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`supply device to the gas concentration sensor selectively,
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`wherein the controller is programmed to maintain the concentration of the standard gas,
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`supply the standard gas to the gas concentration sensor by the standard gas supply device, and on the
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`basis of an output of the gas concentration sensor and the concentration of the standard gas at the
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`moment, calibrate the actually used gas concentration of the gas concentration sensor by controlling
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`the passage control device,
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`US. Patent Application Serial No.: 12/530,555
`Response filed June 25, 2015
`Reply to 0A dated March 25, 2015
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`wherein the controller is connected to at least a first valve which is connected to a first pipe
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`extending out of the incubation chamber, connected to a second valve which is connected to a second
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`pipe connecting the standard gas suppiy device to the second valve, and connected to the gas
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`concentration sensor,
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`wherein the standard gas includes at least carbon dioxide gas, oxygen gas and nitrogen gas.
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`Claim 2 (Previously presented): The incubation apparatus according to claim 1, further
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`including
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`an outside air supply device which supplies outside air to the gas concentration sensor,
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`wherein the outside air supply device is composed of a third valve and a third pipe
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`connecting air to the third valve, and the controller is connected to the third valve and configured to
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`control supply of the outside air to the gas concentration sensor by the third valve of the outside air
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`supply device, and on the basis of an output of the gas concentration sensor at the moment,
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`is
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`configured to perform zero-point calibration of the gas concentration sensor.
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`Claim 3 (Previously presented): The incubation apparatus according to claim 1 or 2, further
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`including a detachable cylinder in which the standard gas is hermetically contained, the standard gas
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`being supplied from the cylinder to the gas concentration sensor by the passage control device.
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`Claim 4 (Previously presented): The incubation apparatus according to claim 1 or 2, further
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`including a connection opening to connect a pipe which supplies the standard gas to the gas
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`concentration sensor.
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`US. Patent Application Serial N0.: 12/530,555
`Response filed June 25, 2015
`.
`Reply to 0A dated March 25, 2015
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`Claim 5 (Previously presented): The incubation apparatus according to claim 1 or 2, wherein
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`the controller is configured to calibrate the actually used gas concentration at preset time intervals.
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`Claim 6 (Previously presented): The incubation apparatus according to claim 1, wherein the
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`gas concentration sensor is capable of detecting the first gas concentration and a third gas
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`concentration in the incubation chamber, and
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`wherein the controller which controls the supply of the first or third gas into the incubation
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`chamber on the basis of an output of the gas concentration sensor;
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`wherein the standard gas and the actually used gas include the first gas, the second gas and
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`the third gas.
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`Claim 7 (Previously presented): The incubation apparatus according to claim 6, wherein the
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`gas concentration sensor including,
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`a first gas concentration sensor which detects the first gas concentration in the incubation
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`chamber, and
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`a third gas concentration sensor which detects the third gas concentration in the incubation
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`chamber.
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`Claim 8 (Previously presented): The incubation apparatus according to claim 7, wherein
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`the first gas concentration sensor and the third gas concentration sensor are connected in series.
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