Application No. 13/521,683
`Response to Ofi’ice Action dated February 20, 2015
`
`Docket No: 061352—0478
`
`AMENDMENTS TO THE CLAIMS
`
`This listing of claims will replace all prior versions, and listings, of claims in the application.
`
`Listing of Claims:
`
`1. (Currently Amended) An amyloid B measurement method comprising the following steps
`
`in the following order:
`
`a sample preparation step in which a sample comprising amyloid B oligomers is placed in a
`
`sample treatment vessel, wherein the sample is an irrigation solution obtained by irrigation of living
`
`tissue;
`
`a concentration step in which a solubilizer that solubilizes amyloid B oligomers is added to
`
`the sample in the sample treatment vessel and an amount of solvent contained in the sample is
`
`reduced without drying and solidifying the sample by a concentration operation to provide a
`
`concentrated sample, wherein the solubilizer is in an effective amount to dissociate amyloid B
`
`oligomers into amyloid B monomers;
`
`a neutralization step in which the concentrated sample is contacted with a neutralizing agent
`
`that neutralizes the solubilizer and provides a neutralized treated sample solution; and
`
`a measurement step in which the amyloid B monomers contained in the neutralized treated
`
`sample solution are quantitatively measured based on an antigen-antibody reaction.
`
`2. (Original) The amyloid B measurement method according to claim 1, further comprising,
`
`prior to the sample preparation step, a sample treatment vessel preparation step in which an additive
`
`to be attached to amyloid B is placed in a sample treatment vessel.
`
`3. (Currently amended) An amyloid B measurement method comprising the following steps
`
`in the following order:
`
`a sample treatment vessel preparation step in which an additive to be attached to amyloid B
`
`oligomers is placed in a sample treatment vessel;
`
`DMMUS 61373473—10613520478
`
`2
`
`
`Response to Ofi’ice Action dated February 20, 2015
`
`Docket No: 061352—0478
`
`AMENDMENTS TO THE CLAIMS
`
`This listing of claims will replace all prior versions, and listings, of claims in the application.
`
`Listing of Claims:
`
`1. (Currently Amended) An amyloid B measurement method comprising the following steps
`
`in the following order:
`
`a sample preparation step in which a sample comprising amyloid B oligomers is placed in a
`
`sample treatment vessel, wherein the sample is an irrigation solution obtained by irrigation of living
`
`tissue;
`
`a concentration step in which a solubilizer that solubilizes amyloid B oligomers is added to
`
`the sample in the sample treatment vessel and an amount of solvent contained in the sample is
`
`reduced without drying and solidifying the sample by a concentration operation to provide a
`
`concentrated sample, wherein the solubilizer is in an effective amount to dissociate amyloid B
`
`oligomers into amyloid B monomers;
`
`a neutralization step in which the concentrated sample is contacted with a neutralizing agent
`
`that neutralizes the solubilizer and provides a neutralized treated sample solution; and
`
`a measurement step in which the amyloid B monomers contained in the neutralized treated
`
`sample solution are quantitatively measured based on an antigen-antibody reaction.
`
`2. (Original) The amyloid B measurement method according to claim 1, further comprising,
`
`prior to the sample preparation step, a sample treatment vessel preparation step in which an additive
`
`to be attached to amyloid B is placed in a sample treatment vessel.
`
`3. (Currently amended) An amyloid B measurement method comprising the following steps
`
`in the following order:
`
`a sample treatment vessel preparation step in which an additive to be attached to amyloid B
`
`oligomers is placed in a sample treatment vessel;
`
`DMMUS 61373473—10613520478
`
`2
`
`
`
`Application No. 13/521,683
`Response to Office Action dated February 20, 2015
`
`Docket No.: 061352-0478
`
`a sample preparation step in which a sample comprising amyloid B oligomers is placed in
`
`the sample treatment vessel, wherein the sample is an irrigation solution obtained by irrigation of
`
`living tissue;
`
`a first concentration step in which the sample in the sample treatment vessel is concentrated;
`
`a second concentration step in which a solubilizer that solubilizes amyloid l3 oligomers is
`
`added to the sample concentrated in the first concentration step and an amount of solvent contained
`
`in the sample is reduced without drying and solidifying the sample by a concentration operation to
`
`provide a concentrated sample, wherein the solubilizer is in an effective amount to dissociate
`
`amyloid B oligomers into amyloid [3 monomers;
`
`a neutralization step in which the concentrated sample is contacted with a neutralizing agent
`
`that neutralizes the solubilizer and provides a neutralized treated sample solution; and
`
`a measurement step in which the amyloid [3 monomers contained in the neutralized treated
`
`sample solution are quantitatively measured based on an antigen-antibody reaction.
`
`4. (Previously Presented) The amyloid [3 measurement method according to claim 1, wherein
`
`the solubilizer is formic acid.
`
`5. (Previously Presented) The amyloid 13 measurement method according to claim 2, wherein
`
`the additive contains formic acid and S—allyl-L-cysteine.
`
`6. (Previously Presented) The amyloid [3 measurement method according to claim 1, wherein
`
`the sample treatment vessel contains a blocking agent.
`
`7. (Original) The amyloid B measurement method according to claim 6, wherein the
`
`blocking agent contains bovine serum albumin.
`
`8. (Previously Presented) The amyloid [3 measurement method according to claim 1, wherein
`
`the sample treatment vessel has an inner wall surface that inhibits adsorption of amyloid B.
`
`DM_US 61373473-10613520478
`
`3
`
`
`
`Application No. 13/521,683
`Response to Office Action dated February 20, 2015
`
`Docket No: 061352-0478
`
`9. (Original) The amyloid [3 measurement method according to claim 3, wherein in the first
`
`concentration step, concentration is performed without insolubilizing amyloid B.
`
`10. (Canceled).
`
`11. (Cancelled)
`
`12. (Previously presented) The amyloid {3 measurement method according to claim 1,
`
`wherein the living tissue is nasal mucosa.
`
`13. (Previously Presented) The amyloid [3 measurement method according to claim 3,
`
`wherein the solubilizer is formic acid.
`
`14. (Previously Presented) The amyloid [3 measurement method according to claim 3,
`
`wherein the additive contains formic acid and S-allyl—L-cysteine.
`
`15. (Previously Presented) The amyloid B measurement method according to claim 3,
`
`wherein the sample treatment vessel contains a blocking agent.
`
`16. (Previously Presented) The amyloid [3 measurement method according to claim 15,
`
`wherein the blocking agent contains bovine serum albumin.
`
`17. (Previously Presented) The amyloid [3 measurement method according to claim 3,
`
`wherein the sample treatment vessel has an inner wall surface that inhibits adsorption of amyloid [3.
`
`18. (Canceled).
`
`19. (Canceled).
`
`DMNUS 61373473-10613520478
`
`4
`
`
`
`Application No. 13/521,683
`Response to Office Action dated February 20, 2015
`
`Docket No.: 061352-0478
`
`20. (Previously Presented) The amyloid B measurement method according to claim 19,
`
`wherein the living tissue is nasal mucosa.
`
`21. (Previously Presented) The amyloid [3 measurement method according to claim 1,
`
`wherein the solubilizer is an organic acid.
`
`22. (Previously Presented) The amyloid [3 measurement method according to claim 1,
`
`wherein the amyloid B monomers are amyloid [3 42 monomers and/or amyloid [3 40 monomers.
`
`23. (Previously Presented) The amyloid B measurement method according to claim 1,
`
`wherein in the concentration step, the solubilizer is added to the sample to obtain a mixture and the
`
`mixture is subjected to the concentration operation to reduce the amount of solvent contained in the
`
`sample.
`
`24. (Currently Amended) An amyloid [3 measurement method comprising the following
`
`steps in the following order:
`
`a sample preparation step in which a sample comprising amyloid [3 oligomers is placed in a
`
`sample treatment vessel;
`
`a concentration step in which a solubilizer that solubilizes amyloid [3 oligomers is added to
`
`the sample in the sample treatment vessel and an amount of solvent contained in the sample is
`
`reduced without drying and solidifying the sample by a concentration operation to provide a
`
`concentrated sample, wherein the solubilizer is an organic acid in an amount effective to dissociate
`
`amyloid B oligomers into amyloid B monomers;
`
`a neutralization step in which the concentrated sample is contacted with a neutralizing agent
`
`that neutralizes the solubilizer and provides a neutralized treated sample solution; and
`
`a measurement step in which the amyloid B monomers contained in the neutralized treated
`
`sample solution are quantitatively measured based on an antigen-antibody reaction.
`
`DM_US 61373473—1 0613520478
`
`5
`
`
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