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`dos hrcvct 5
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`off-mempéen
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`DESCRIPTION JP2007315879
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`[0001]
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`The present invention relates to an optical analysis device and an optical analysis system for
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`optically analyzing a biological fluid, more particularly, the analysis device to be used for
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`component measurement of a biological fluid with an optical analyzer on the transport means of
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`the structure and the liquid sample of the chamber in.
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`[0002]
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`Conventionally, blood analyzing disk is used which is collected therein as a liquid sample, while
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`rotating the analytical disk around the axis, using optical scanning techniques of the optical disc
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`apparatus, the analyzer for analyzing the properties of the blood some (for example, see Patent
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`Document 1).
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`[0003]
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`As shown in the exploded perspective view in FIG. 1 7, the analysis disc 100 includes a base
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`substrate 1 1 7 concentric or spiral tracks 101 are present, to form a groove serving as will flow
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`path made of blood is collected transported a spacer substrate 1 18, these upper and stuck over
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`cover 1 1 9 forming the inlet and the air outlet of the blood and is constituted.
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`[0004]
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`Plan View of FIG. 18, there is shown an enlarged one of the flow channels provided in the
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`analysis disk 100.
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`Analytical disc 100, collects a certain amount of blood, blood in the first chamber 200 to be
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`separated into blood cells and plasma components are disposed outside the axis relative to the
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`first chamber 200, a first It and a capillary 202 which connects the second chamber 201 of the
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`plasma component separated by the chamber 200 is transported, a first chamber 200 and
`second chamber 201.
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`[0005]
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`The analysis operation using such analysis disk, with the blood 208 is filled from the first inlet
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`204 formed in the chamber 200, blood was collected into the first chamber 200 in such a pipette
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`and analyzed It rotates the use disk 100.
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`Then the blood is a state that does not exceed the apex 202a of the capillary 202, that is,
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`centrifugal separation is performed in a state held in the first chamber 200.
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`[0006]
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`After centrifugation, the cell stops the rotation of the analysis disc 100, the centrifugal separated
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`plasma component is beyond the apex 202a of the capillary by capillary action, and is
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`transported to the entrance of the second chamber 201.
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`[0007]
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`Then, again, is rotated for analysis disc 100, by centrifugal force and capillary action force, the
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`separated plasma component is transferred from the first chamber 200 into the second chamber
`20 1 .
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`In the second chamber 20 1, it is possible to hold the reagent which develops a color by reacting
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`with the specific substance that want to analyze in plasma.
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`As a method of applying a reagent, before pasting the upper cover 1 1 9 on top of the spacer
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`substrate 1 18, the second chamber 201 parts of the upper cover 1 19 is carried out by making it
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`dropwise reagent drying.
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`After the dried reagent is uniformly applied to the wall surface of the second chamber of the
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`upper cover 1 19, I paste upper cover 1 19 on top of the spacer substrate 1 18.
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`[0008]
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`After color reaction the transfer is completed in the second chamber 201 has occurred, while
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`rotating the analytical disk 100, the outer circumference from the inner circumference or toward
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`the inner periphery from the outer periphery, along the track 101 of the By irradiating light to
`scan the second chamber 20 1.
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`And by detecting the transmitted light obtained from the second chamber 201, it is possible to
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`detect the amount of a specific substance in the blood plasma component which has reacted with
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`said reagent.
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`[0009]
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`In addition, by changing the types of reagents to be held in the second chamber, the analysis of
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`other materials in the plasma components are possible, also, it is possible to detect by using a
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`reflection light not only transmitted light .
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`Further, the above flow path, although an example that is constructed from the first only in the
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`second chamber, is not limited to this, and further, as provided by concatenating the more the
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`chamber after the second chamber It may be constructed.
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`Hei 10—504397 JP
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`[0010]
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`Meanwhile, in an optical analyzer as described above, the plasma component, which is
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`centrifuged at the first chamber 200, a predetermined amount necessary for analysis in the
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`second chamber 201, have to be reliably transported through the capillary 202 shall.
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`[001E
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`However, in the conventional analyzer, for miniaturization of the analysis disc, as far as possible
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`the thickness of the first chamber 200, partially Although we want to thin at all, the thickness of
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`the first chamber 200 less difference between the thickness of the capillary 202, the capillary
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`force generated between the first chamber 200 and the capillary 202 becomes small, no
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`sufficient driving force is obtained for transferring the plasma component, the amount of
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`required there is a problem that can not be transported blood components into the second
`chamber 20 1.
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`[001%
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`In addition, first chamber 20 1 is not a separation chamber, just when the reagent is a coated
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`chamber, mixes with injected or transported by getting the blood, when the reagent is dissolved
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`in the blood, the corners of all means in the chamber Part I can reagent is a bias uneven (end).
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`This means that when dropping reagents is dried, since the biased to absolutely corners reagents
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`wall of the first chamber 201 (end), upon dissolution of the infusion solution, the corners of the
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`chamber (end) The concentration of the reaction solution becomes high in, there is a problem
`that not uniform.
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`[001%
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`To prevent this, as the region of the corners of the chamber (end) is small, and although we want
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`to thin as possible the thickness of the first chamber 200, for transferring blood to the second
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`chamber 201 For but it can not provide sufficient transfer driving forces, since reliable agitation
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`utilizing the transport propulsion force is insufficient, even when transferred even into the
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`second chamber 201, and inhibits the reaction in the subsequent chambers and sisters, there is a
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`problem that can not be highly accurate analysis.
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`[0014]
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`The present invention is intended to solve the above problems, for example, even when the
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`thickness of the chamber is small, reliable as well as to transfer driving forces, such as solutions
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`capable of transporting is obtained reagent can be uniformly dissolved and an object thereof is to
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`provide an optical analyzing device of the optical analysis system.
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`[0015
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`In order to solve the above problems, an optical analyzing device of the present invention, the
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`liquid sample by analyzing the light that the liquid sample is emitted based on the light irradiated
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`to the liquid sample contained in a chamber provided inside In the optical analysis device for
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`analyzing the disposed around the rotation axis of the optical analysis device, with the first
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`chamber for a liquid sample constructed injectable to, with respect to the rotation axis than the
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`first chamber is located outside Te is connected with the first chamber and the capillary, a
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`second chamber for the liquid sample injected into the first chamber is constructed acceptably
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`through the capillary, with the said first chamber, a first region having the wherein the rotary
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`shaft space is long length in a direction parallel to the more capillary upstream of the liquid
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`sample flow path, and the length in a direction parallel to the rotary shaft on the connecting
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`portion side of the capillary is longer than the capillary, and it has a second region having a
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`space shorter length in the direction parallel to the rotational axis than the first region, it is
`characterized.
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`[001m
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`The present invention is in the first chamber, in the above and the first region a second region,
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`the difference in the direction of length parallel to the rotation axis, characterized in that a
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`50 ,LL m least 2mm less.
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`[001W
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`The present invention, in the first chamber, the volume of the first region, characterized in that it
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`is more than the volume of said capillary.
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`[0018]
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`The present invention, in the first chamber, characterized in that the reagent that reacts with the
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`liquid sample to the wall surface forming the second region is applied.
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`Furthermore, the present invention, the second region is formed by bonding two substrates, the
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`liquid sample cross section in the vertical direction of the substrate in the direction of inflow,
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`characterized in that with a concave shape.
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`[0019]
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`Furthermore, the present invention, the concave shape forms a semi—circular shape, the second
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`region is characterized by formed by including the wall of the semi—cylindrical.
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`[0020]
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`The present invention, in the second area has a ground glass-like concave and convex portions
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`on at least part of the surface of the substrate having the concave shape, height difference in the
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`concavo-convex portion is, 0.01mm or 0.5mm or less and characterized in that it is.
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`[002E
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`The present invention, in the second region, the surface of the substrate having the concave
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`shape, characterized in that it has two or more grooves.
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`[002%
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`The present invention, in the second region, the surface of the substrate having the concave
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`shape, characterized in that it has a flow substantially parallel to the two or more grooves in the
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`liquid sample.
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`[002$
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`The present invention, in the second region, wherein the substantially parallel grooves, at the
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`following intervals 2mm, at 1mm or less, the depth of the groove, characterized in that it is
`0.05mm or 1mm or less .
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`[0024]
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`The present invention, in the second region, the cross—sectional shape in the direction of the
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`substrate which the liquid sample flows, characterized in that it has a semicircular or semi-
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`elliptical shape on the downstream side.
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`[0025
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`Furthermore, the present invention, the optical analysis device is detachable test strip from the
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`analyzer I is characterized in that said capillary and said chamber are provided in the test piece.
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`[002m
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`Also, the A analyzer for optical analysis device is mounted, said analysis device comprising a
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`rotational drive means for rotating around the axis, wherein the optical analysis device in which
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`the liquid sample is accommodated in the first chamber the plasma component of said liquid
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`sample by stopping the rotation to transport the up capillaries, wherein the to be transferred to
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`the second chamber a plasma component of said liquid sample by rotating again.
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`[002W
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`In addition, the reagent is characterized in that it is a reagent for aggregating cholesterol
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`components other than HDL-C in the plasma component.
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`[0028]
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`According to the optical analyzing device of the present invention as described above, when the
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`thickness of the first chamber 200 is thin, the although the difference in thickness between the
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`thickness and the capillary 202 of the first chamber 200 is small because the required amount of
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`and inability to generate sufficient capillary force, such as solutions can be reliably transferred
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`into the second chamber, since the capillary force is insufficient, to the problem that it is
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`impossible to uniformly stirred with a reagent in the chamber, an auxiliary it is possible to give
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`emitted propulsive transfer force, and can reliably transfer.
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`[0029]
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`Also, since a sufficient driving force for reliably perform the transfer of solution is obtained, upon
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`transfer, stirring action occurs, will be able to uniformly stir the reagent, it is possible to improve
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`the analytical system for later there is an effect that.
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`[0030]
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`When the dripping reagents is dried, because the reagent can be uniformly coated without biased
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`to a corner portion of the wall of the first chamber 20 1 (end), when mixed with a solution,
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`uniform agitation can be effectively It has also.
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`[003M
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`The following, for the optical analysis device of the present invention, it will be described with
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`reference to the accompanying drawings in detail the embodiment.
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`[003m
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`(Embodiment 1)
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`It shows a block diagram of the analyzer of Embodiment 1 of the present invention. FIG.
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`At the bottom of the analyzer, an optical pickup 104 which irradiates light to the analyzing
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`device 100, the traverse motor 105 for moving the optical pickup 104 in the radial direction of
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`the analysis device, and for driving rotating the analysis device 100 It has placed the spindle
`motor 106.
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`These, by calculating the information read track 101 obtained by the optical pickup 104 by the
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`signal processing circuit 108, CPU109, is driven through a servo control circuit 107.
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`[0033
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`At the top of the analyzer of the laser light irradiated from the optical pickup 104, a
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`photodetector 103 for detecting the transmitted light 102 having passed through the analyzing
`device 100 is located.
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`[0034]
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`Signal detected by the optical detector 103, A / D converter 1 1 1 is A / D converter, A / D
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`converted data is signal processed by the signal processing circuit 1 1 5, a specific substance in a
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`liquid sample, for example blood Other concentrations and amounts, depending on the purpose
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`of analysis, the shape of the material is calculated and the size.
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`Memory 1 1 2 can not hold a table for converting the value calculated by the signal processing
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`circuit 1 1 5 to a value such as the concentration of a particular substance, or to store values
`obtained concentration.
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`such
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`The obtained analytical data, which is output to the display unit 1 20, which controlled by
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`CPU1 14 is performed.
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`[003a
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`Now, in the present embodiment, uses a structure analyzing device shown in FIG. 17, the
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`structure of the flow path is different from that shown in Figure 18, the channel structure shown
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`in Figure 2 It described an example of the device for optical analysis that has to.
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`[003%
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`Channel structure shown in Figure 2 is intended for measuring the good cholesterol, called HDL-
`C.
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`[003W
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`Specific structure includes a first chamber 200 having an inlet 204 for injecting a liquid sample,
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`a second chamber 201 which is connected with the first chamber 200 and the capillary 202, the
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`second chamber 201 and the capillary 205 a third chamber 203 which is connected in, and
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`further, and a fourth chamber 206. which is connected with the third chamber 203 and the
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`capillary 207.
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`The third chamber 203, the fourth chamber 206, the reagents necessary for analysis of HDL—C is
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`applied.
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`[0038]
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`Below along the flow of analyzing HDL—C, the analyzer of the present embodiment will be
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`described a basic operation for performing analysis with high accuracy.
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`[0039]
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`First, injecting the injection port 204 from the blood 208 of the analysis device 100, it is
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`mounted in the analyzer of the analysis device 100 in a state that satisfies a first chamber 200 in
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`the blood, spin-up to be performed in the conventional optical device apparatus Processing I do.
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`[0040]
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`After this, it does centrifugation process of separating blood into a plasma component 300 and
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`the blood cell component 301.
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`Centrifugation process, for example, at a rotational speed of 4000~6000rpm, for 2-3 minutes,
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`rotating the analyzing device 100.
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`[0041]
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`After the predetermined time has elapsed, and stopping the rotation of the analysis device 100, a
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`plasma component 300 separated in the first chamber 200 is transferred through the capillary
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`202, the flow advances to the entrance of the second chamber 201.
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`Where it is rotated again analyzing device 100 at a rotational speed of 1000~3000rpm, the
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`plasma component flows into the second chamber 201, and satisfy the second chamber 201 in
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`the plasma component 300.
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`Volume of the second chamber 201 is designed smaller than the volume of blood plasma
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`component to be separated in the first chamber 200.
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`Therefore, the second chamber 201 so fill in the plasma component can be metered amount of
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`plasma components (quantitative) (Figure 3).
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`[0042]
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`Again, progress and stop the rotation of the analysis device, a second plasma component 300 of
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`the supernatant that is retained on the inner peripheral side of the chamber 20 1 to flow from the
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`second chamber 201 to the capillary 205, to the entrance of the third chamber 203 to.
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`Then again, rotating the analysis device 100 at a rotational speed of 1000~6000rpm, plasma
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`components 300 are transferred from the capillary 205 to the third chamber 203, and satisfy the
`third chamber 203.
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`[0043]
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`In the third chamber 203, the plasma component is reacted which has been transferred to a pre—
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`arranged reagent chambers.
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`The third chamber 203, it is possible to place a reagent which produces agglutination reaction
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`with the cholesterol component of the non-HDL—C in the plasma component 300.
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`The reason for placing such agglutination reagent, in later analysis, is to remove the cholesterol
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`component of the non—HDL-C to inhibit the optical analysis of HDL-C.
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`A certain time, when the rotation of the analysis device be stopped or low rotation, as shown in
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`Fig. 5, aggregates 303 are produced in the third chamber 203.
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`[0044]
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`After a certain time, the analysis device again, 3—10 minutes approximately at a rotation speed of
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`3000~6000rpm, by rotating, moves the resulting aggregates as shown in Figure 6 in the outer
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`peripheral direction of the device, aggregates 303 do centrifugation.
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`[0045]
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`In follow to the above—mentioned transfer method, transfer the plasma component 303 other
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`than the agglomerate 303 to a fourth chamber 206 (Figure 7).
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`The fourth chamber and reacts with HDL-C and is previously holding the reagent 304 that
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`produces a color reaction, moves the optical pickup 104, an optical detector 103 to a fourth
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`chamber 206, the optical pickup 104 detecting the transmitted light by the photo detector 103 is
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`irradiated with laser to perform the absorbance measurements.
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`[0046]
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`Memory 1 1 2 stores a table relating the absorbance and HDL—C levels, the analyzer, the output to
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`the display unit 1 20 reads out the HDL-C concentration corresponding to the absorbance was
`detected.
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`[0047]
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`Here, in the above—mentioned series of HDL-C concentration measurement, the characteristic
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`configuration of this embodiment will be specifically described.
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`In the present embodiment, in the second chamber 201, wherein the generating a sufficient
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`transfer driving force for carrying out a reliable transport.
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`[0048]
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`For what kind of things with sufficient capillary force that can transport the liquid, it will be
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`explained with reference to FIG. 1 1.
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`[0049]
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`1 1, plasma is transferred into the second chamber 201 is a plan view showing a second chamber
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`201 when it is filled with the plasma component.
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`Advanced when stopping the rotation of the analysis device, a second plasma component 300 of
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`the supernatant that is retained on the inner peripheral side of the chamber 20 1 to flow from the
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`second chamber 201 to the capillary 205, to the entrance 412 of the third chamber 203 to.
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`[0050]
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`Progress in this case, the plasma component is not always necessary to reach to the entrance
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`4 1 2 of the third chamber 203, from the outermost surface 413 of the second chamber, within
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`the region 4 1 1 until the inlet 412 of the third chamber 203 and if it was, again, when rotating
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`the analysis device 100 at a rotational speed of 1000~6000rpm, plasma components 300 are
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`transferred from the capillary 205 to the third chamber 203, it is possible to fill the third
`chamber 203 .
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`[005E
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`Therefore, when stopping the rotation of the analysis device, the plasma component 300 of the
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`second chamber 201 is at least, ensure that the transfer propelled enough to proceed to within
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`region 41 1 beyond the outermost peripheral surface 413 of the second chamber force becomes
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`necessary.
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`[005m
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`8, which has expanded the second chamber 20 1 in this embodiment is a cross—sectional view
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`taken along line A—A of FIG.
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`In Figure 8, and it shows a state before the plasma is transferred to second chamber 201.
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`The second chamber 20 1 includes a first region 401 greater thickness than at least the capillary
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`205 on the upstream side, on the side of the capillary 205 are connected, in the capillary 205 or
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`more in thickness, and the first than regions 401 i and a second region 402 having a small
`thickness.
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`That is, the axial direction of the thickness of the second chamber 201, the side closer to the
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`capillary 205 which is downstream, and provided with a step such that shallow, liquid and has a
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`cavity 4 1 7 to be flowed into it.
`
`[005$
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`Then, it will be explained dimensional relationship.
`
`Axial dimension 406 of the first region 401 is about 600 JLL m, the dimensions of the axial
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`dimension 407 of the second region 402 is about 200 p m.
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`Axial dimension 403 of the capillary 205 is about 100 ,le m, the axial dimension 403 of the
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`capillary 205 is approximately 50 [.L m.
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`The dimensional 408 transport direction (track direction) of the first region 401 is about 2mm,
`
`the size 409 of the transport direction (track direction) of the second region 402 is about 8mm,
`and.
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`[0054]
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`Therefore, by the dimensions of such a thickness, when it is allowed to flow into the liquid, in
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`addition to the capillary force 4 1 8 is generated by the difference in size of the second region 402
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`and the capillary 205 of the second chamber 202, the same chamber even in the 201, since the
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`first region 401 the capillary force due to the difference in size of the second region 402 (force
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`caused by capillarity) 4 10 is generated, it is possible to generate a more potent specific transfer
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`propulsion.
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`9, the analyzing device 100 when it is rotated at the rotational speed of 1000~3000rpm, is a
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`representation of how the second chamber 201 is filled with the plasma component.
`
`[0055
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`In the second chamber 20 1, after the blood plasma component has been transferred in advance
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`arranged reagent chambers is reacted again, and stopping the rotation of the analysis device, and
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`held on the inner peripheral side of the second chamber 201 plasma component 300 of the
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`supernatant flows from the second chamber 201 to the capillary 205, it will proceed to the
`entrance of the third chamber 203.
`
`[005m
`
`In this case, the capillary force 4 10 acting on the plasma component 405, as shown in Figure 10,
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`in the second chamber 201, between the first region 401 and second region 402 acts in the
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`direction of the arrow , it becomes transfer driving force.
`
`This transfer driving force, in order to generate the effect of strengthening the capillary force
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`4 18 acting between the second region 402 and the capillary 205, the plasma component 405 is
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`surely sucked into the capillary 205.
`
`[OOSfl
`
`In other words, this capillary forces 4 10, when stopping the rotation of the analysis device, the
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`plasma component 300 of the second chamber 201 flows through the capillary 205, ensures
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`that the region 41 1 beyond the outermost peripheral surface 4 13 of the second chamber This is
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`to be able to generate sufficient capillary force enough to progress up to the range.
`
`[0058]
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`Then, it will be briefly described the volume of the first region 401.
`
`When the analysis device is stopped, the plasma component 300, which is flowing into the
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`capillary 205, during the rotation of the analysis device
`
`Also on the inner peripheral side area of
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`the (pre-stop) a second region 402 where the plasma
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`component was not met, and (not shown) flows through the transfer propulsion.
`
`Therefore, the volume of the first plasma component that is filled in the region 401 during
`
`rotation of the analysis device can, in the second region 402 of the plasma component at the
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`time of rotation (before stopping) of the analysis device has not been met the volume which has
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`flowed into the inner peripheral side region, and may the more than the sum of the volume of the
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`plasma component 300 of the capillary 205 has progressed to the outermost peripheral surface
`4 13 of the second chamber.
`
`In other words, the volume of the first region may be any size of capacity to satisfy the above
`
`conditions, it is desirable to be at least more than the volume of said capillary.
`
`However, if the volume of the first region of the volume smaller than the above condition, when
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`the stop analyzing device, since the plasma component 300 will be unable to proceed to the
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`outermost peripheral surface 4 13 of the second chamber in the capillary 205, and analyzes by
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`respin iodine devices, results in the inability to transfer to the third chamber 203.
`
`[0059]
`
`In the present embodiment, to generate the transfer propulsion force by the axial dimension 406
`
`of the first region 40 1 a difference between the dimension of the axial dimension 407 of the
`
`second region 402 to about 400 ,u m In that, but the structure of the thickness of the analyzing
`
`device, the dimensional difference is, 50 ,u m or 2mm or less (preferably, 300 [,L m or 500 p m or
`
`less) if, it is possible to generate sufficient capillary force, a second The plasma component 300
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`of the chamber 201 ensures that it can be transported through the capillary 205 to the third
`chamber 203.
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`

`[0060]
`
`Furthermore, the dimensional difference is 50 ,u m or less, a plasma component 300 of the
`
`second chamber 201, since a sufficient capillary force only to transfer through the capillary 205
`
`to the third chamber 203 is not generated, from the viewpoint of occurrence of the transport
`
`propulsion When the mill was not obtained much effect in experiments.
`
`[006M
`
`In addition, the theory, although it is possible to to 2mm larger size difference, thin as possible
`
`the thickness of the analyzing device itself and the small of the analysis device capable of
`
`measuring a small amount of use as long as the plasma component 300 can also be it is, which is
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`advantageous in reduction and cost aspects of the measurement time.
`
`Therefore, the analysis device having a chamber of 2mm larger size difference is not very
`realistic.
`
`Furthermore, by utilizing the optical disk of an optical disk apparatus as the base substrate of the
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`intact analysis device, if the upper cover has a chamber 2mm larger size difference, the thickness
`
`of the upper cover can be considered to thin the wall thickness The now greater than about
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`3~4mm, the thickness of the assay device itself, since what is in excess of about 5mm, it is
`evident that it is not realistic.
`
`[006m
`
`Incidentally, the capillary force generated is slightly affected by the surface treatment of each of
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`the inner wall of the capillary and chamber, as the capillary force is high hydrophilicity is higher,
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`is less affected by the cross—sectional shape of the chamber of the capillary.
`
`[006$
`
`In this embodiment, the shape of the external appearance of an optical analysis device, which is
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`

`convenient manner is a circular device is not limited to a circular device if a rotatable device,
`
`including those of the rectangular or oval shape, etc. any shape.
`
`[0064]
`
`In this embodiment, the optical analyzer, as shown in Figure 1, the analyzer which applies the
`
`optical scanning technique of the optical disc apparatus, an information area (track information
`
`or address information for controlling the reading of the rotation and the signal ) was the optical
`
`analysis device having, but not necessarily need thereof.
`
`Structure of an optical analyzing device may be a data area for controlling the reading of the
`
`rotation and the signal (track information or address information) has no detachably configured
`
`test strip (panel—like), Analysis a rotatable plate (not shown), or may be a structure such as may
`
`be attached to the test strip directly.
`
`[006a
`
`In this embodiment, the inlet 204 is provided on the surface upper portion of the first chamber
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`200, but the inlet, that the specimen is formed on the end face of the center side of the rotating
`
`(not shown) By, after the spotting, a liquid sample may be caused to reliably transfer and
`
`retention to the reagent side by the centrifugal force during the test.
`
`[006m
`
`As described above, according to the first embodiment, when performing the transfer from
`
`chamber to chamber, the amount required for analysis can be reliably transported, the analytical
`
`accuracy of the reliability of subsequent HDL—C and it can be improved.
`
`[OOGfl
`
`(Embodiment 2)
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`Next, as a second embodiment of the present invention will be described an example in which the
`
`reagents necessary for the analysis were held in the second chamber 201 can be dissolved
`
`uniformly and evenly.
`
`Optical analysis system and configuration of the analysis device used in the second embodiment
`
`is the same as the configuration described in the foregoing first embodiment.
`
`A is different from the first embodiment, also in the second chamber 201 of the analysis device,
`
`(including agents which hemolysis of blood cells) precipitating the components other than HDL—C
`
`is different from that by pre-holding the reagent .
`
`Hereinafter, mainly the differences will be described the operation of the analyzer of the present
`embodiment.
`
`[0068]
`
`Figure 4 in which the plasma component is showing a state of a time that is transferred to the
`
`second chamber, would be reacted with the reagent that is applied to the second chamber.
`
`[0069]
`
`1 2 is obtained by enlarging the second chamber 201 in this embodiment is a cross-sectional view
`
`taken along line A—A of FIG.
`
`The inner wall area of
`
`the first area 401 a small second region 402 in thickness than that by
`
`drying the dropped reagent 4 14, the reagent 414 is applied.
`
`This, although there is a possibility that also carries the reagents 4 14 in the first region 401, as
`
`described in later, when dropped reagent 4 14 is dried, inevitably reagents by surface tension the
`
`property that gather in corners, it means that the lack of uniformity during the dissolution of the
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`plasma components, as a countermeasure, the inner wall to the reagents 414 in the region of the
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`second region 402 narrow shallow areas of the corners in the embodiment it is preferable to
`
`carrying.
`
`[0070]
`
`Here, by the shape of the wall of the second chamber 201, how much to affect the reaction, it
`
`was an experiment in the following manner.
`
`[OOTH
`
`The walls of the second chamber 201 giving the process into a predetermined shape, after
`
`coating and drying the precipitate reagent to the chamber 201 (sodium phosphotungstic acid
`
`and magnesium chloride), and then transferring the plasma component, and a non-through
`
`reaction with a precipitating agent After precipitation of HDL—C components, it was performed by
`
`measuring or non—HDL-C component is included much above Shizunai.
`
`[007%
`
`For the shape of various wall (inner surface), as shown in Figure 16a~d, raw wall, ground—glass
`
`wall, the wall having a five grooves, the wall having a 10 grooves, the recess in the center wall is
`
`provided, and respectively with the five types.
`
`Figure 20, for each wall of these five types is the result of whether the reaction is carried out
`
`reliably, was performed by measuring the non-HDL—C component (LDL) to be precipitated with
`
`the precipitating reagent, Experimental results 1 and Experiment Result 2 is the result of an
`
`experiment with two original samples with different concentrations of the sample liquid.
`
`[007$
`
`In the chambers subjected to machining into the shape of various wall, after precipitation, the
`
`recovered upper static, using an automated analyzer "Hitachi 7020" it was determined TC
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`concentration, the LDL concentration.
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`[0074]
`
`In experiments 1, the wall surfaces of the raw, with a guide by focusing on LDL as a
`
`representative of the non —HDL components of the original sample of 103mg / d1, LDL that is not
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`precipitated by the precipitation reaction process, 3mg / dl present to.
`
`In contrast, ground glass wall, the wall provided with five grooves, the wall having a 10 grooves,
`
`the wall having a recess in the center, LDL that is not precipitated by the precipitation reaction
`
`process, 0mg It has become a / dl.
`
`[0075
`
`Also, the experimental results 2, the wall of the raw, paying attentio